From expert task to lab routine Membrane protein purification and analysis simplified
Inhalte des Web-Seminars
Dauer: ~60 min
Sprache: Englisch
Referenten:
Stefano Manduzio, Field Application Specialist
Frederik Huhn, Junior Application Specialist
Matthias Ludwig, Senior Application Specialist
More than 60% of approved drugs act directly on membrane proteins—targets that underpin many high‑value and widely used therapeutics. Yet only about 2% of membrane protein targets have been fully characterized, despite their importance in treating cancer, immune and metabolic disorders, allergies, cardiovascular disease, and neurological conditions.
The challenge: membrane proteins remain some of the most challenging targets to work with experimentally. As GPCRs, ion channels, transporters, and other surface receptors, they require native stability and functional integrity to generate meaningful data. Traditional purification workflows are labor-intensive, highly manual, and often dependent on multi‑day procedures in dedicated environments such as cold rooms.
In this webinar, we introduce a streamlined solution to these bottlenecks. You will learn how membrane protein purification can be simplified and standardized through an automated, detergent‑free workflow using compact, lab‑ready automation that integrates seamlessly into existing research settings. A practical demonstration will show how high‑quality membrane proteins can be purified in under three hours and prepared for downstream stability analyses such as thermal shift assays (TSA).
The purification method presented preserves key lipid bilayer components to maintain native protein structure and function. Using the detergent‑free PlateX™ assay with the CyBio FeliX, eight different membrane protein types were purified with excellent results. Subsequent qPCR‑based TSA enables sensitive, real‑time stability measurements of protein stability through fluorescence detection. The method supports scalable screening of buffers, ligands, and stabilizing additives. Digital miniaturization down to the picoliter scale with the PULSEspencer R helps saving valuable samples, and ensures fast, precise reagent dispensing, ideal for sensitive dyes like CPM.
Lernziele:
After the webinar, you will have a clear understanding of the following aspects:
- Discover how to purify stable native membrane proteins using polymers in an automated 3-hours workflow
- Learn how to apply thermal shift analysis with qPCR successfully to membrane protein analysis
- Explore how to miniaturize samples with digital dispensing in the 8 pL…200 µL
- Ask our experts questions and benefit from their knowledge
Zielgruppen:
- Researchers in structural biology, proteomics, and biophysics
- Lab managers of core facility teams
- Managers of CROs
- Managers of discovery groups in the pharmaceutical and biotechnology sector
- Researchers and lab managers of early research in pharma and biopharma
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