rapidSTRIPE Borrelia Assay

Features

  • Rapid detection of Borrelia in ticks
  • Ready-to-use kit includes all consumables and reagents needed for PCR and detection
  • Based on a highly specific, rapid amplification/hybridization reaction (RAH)
  • Detection performed on a stable Lateral Flow Strip suitable for archiving

Product Info

The rapidSTRIPE Borrelia Assay can be used for direct detection of Borrelia from DNA isolated from ticks. The kit contains all of the consumables and reagents needed for the combined amplification hybridization reaction and for final detection on a Lateral Flow Strip. The reaction format thus allows specific detection of Borrelia DNA and prevents false positive results due to mispriming. Protocols for detection are available either for using rapidPCR or PCR. In addition, kits that optimize nucleic acid extraction from ticks are offered as part of the blackPREP product line.

Order Info

Order number

Quantity

845-IS-1002025

24 reactions

845-IS-1002050

48 reactions

Specifications

Detection time:

Approx. 10 – 20 minutes

Time for cDNA synthesis, amplification and hybridization:

  • cDNA synthesis: approx. 30 minutes
  • rapidPCR (SpeedCycler ²): approx. 50 minutes
  • Standard PCR: Depends on thermal cycler, approx. 2 – 2.5 hours

Sensitivity:

Comparable to TaqMan® real-time PCR

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