innuTaq DNA Polymerase

特長

  • Optimal for routine PCR
  • Convenient and reliable amplification
  • Extremely thermostable
  • Best reproducible results

製品インフォメーション

Analytik Jena‘s innuTaq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E.coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5’ → 3’ DNA polymerase activity. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95 °C). It is recommended for use in routine PCR reactions.

オーダーインフォメーション

Order number

Quantity

845-EZ-1000500

500 Units

仕様

濃度:

5 U/µl

(One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C.)

酵素保存バッファ:

20 mM Tris-HCl pH 8.0; 100 mM KCl; 1 mM DTT; 0.1 mM EDTA; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % Glycerol

PCRバッファ:

  • 10× PCR Buffer with KCl; 100 mM Tris-HCl (pH 8.8 at 25 °C); 500 mM KCl; 0.8 % Nonidet P40
  • 10× PCR Buffer with (NH4)2SO4; 750 mM Tris-HCl (pH 8.8 at 25 °C); 200 mM (NH4)2SO4; 0.1 % Tween 20

PS-Mg2+ solution:

MgCl2 stock solution, 25 mM

ダウンロード

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(e.g. Manual, Short Manual)

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