qTOWER³ auto

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Features

qTOWER3 auto - Real-Time PCR meets Automation

  • Convenient: 12 available Color, FRET and Protein Modules can be exchanged or reconditioned within 5 minutes
  • Future-proof: Adaptable to new applications at any time
  • Robust: Patented high performance optical system with a long-term warranty of 10 years
  • Fast: Heating rates of 8 °C/sec and cooling rates of 6 °C/sec
  • Unrivaled: Ideal homogeneity and temperature control accuracy (± 0.1 °C)
  • Comfortable: Automated plate handling by moveable sample tray
  • Intelligent: Decoupled electronic module with 10 m cable for peripheral positioning
  • Universal: Automatic data evaluation form a simple overview of Ct
    values up to ddCT method and multi plate analysis
  • Transparent: Reliable data management based on different export
    functions and a LIMS transfer file
  • Efficient: Short scan times of 6 seconds for the entire plate, regardless of the number of dyes detected
  • Innovative: Novel light source with 4 robust LEDs – no preheating
  • Brillant: Ideal illumination and excitation of all 96 probe without
    fringe effect

Product Info

Automation Made Easy
In addition to the externally accessible sample tray, plate handling is also automated in qTOWER³ auto. The 96 well microplate is positioned precisely within the block and, following amplification, is unloaded just as efficiently. Visualizing a continuous workflow is easy and reliable. The concept of automated real-time PCR accommodates a variety of integration strategies. The length of the cable for the decoupled electronics module is up to 10 m, making it easy to integrate from a decentralized location. The size of the rest of the instrument is then only just 27.5 cm x 46 cm x 31 cm (W x H x D), and the height of the sample tray (minimum = 12 cm) is easy to adjust. The cycler unit can be positioned next to the liquid handling system to save space, in which case the plate is loaded using an external robotic arm (as used in the CyBi®-Carry) or by directly connecting the sample tray to the liquid handling station.

Optimum Data Handling

The system automatically carries out the following steps as a basis for all further analyses: performs threshold and Ct determinations on each sample, generates standard curves, and determines PCR efficiency. Depending on the assay and the desired level of information, these are followed immediately by the below shown methods:

  • Absolute or relative quantification
  • ddCt method with or without efficiency
  • Determination of point mutations using probe based, allelic discrimination (genotyping)
  • POS/NEG analysis by endpoint determination
  • Melting curves and protein analysis
  • Multi gene or multi plate analysis respectively

High-Performance Optical System
The automated cycler is every bit as powerful as the comparable stand-alone version of qTOWER³. The heart of the high-performance optical system (FOS - Fiber Optic System) is the patented, optical shuttle with 8 optic fibers, which optimizes excitation for each individual sample, while detecting the emitted fluorescence signals with extraordinary homogeneity. The system is rounded out by its novel light source, which consists of 4 long-lasting, intense LEDs in red, green, blue and white (RGBW), delivering highly selective extinction as a function of the selected dye.

qTOWER³ auto is equipped with supporting filter modules that include a finely tuned set of excitation and emission filters. Depending on the application, customers can choose from 6 Color Modules, 5 FRET Modules and one Protein Module, up to 6 of which can be installed in the instrument. This allows the system to carry out highly sophisticated multiplex applications, from the blue to near infrared range. The system reads out the 96 well plate in just 6 seconds, regardless of the number of filter modules.

Modern Silver Sample Block
The sample block is the foundation of any PCR or real-time PCR system. qTOWER³ auto uses a combination of high-quality silver and a gold coating to generate heating rates of up to 8 °C/ sec. At the same time, the system also delivers excellent control accuracy to within just
± 0.1 °C of the target temperature. The optimized temperature control algorithm makes the thermocycler the ideal system for quantitative and qualitative real-time PCR applications. In addition, qTOWER³ auto also comes equipped with a motorized heating lid that can be set to temperatures of up to 110 °C. The optimum, automatic contact pressure of the heating lid perfectly seals the PCR plates used.

Technical Data

Thermal block

Sample block

Silver sample block with gold-coating

Block capacity

96 Well Microplates (0.2 ml)

Sample size

10 – 60 μl

Heating

Up to 8 °C/sec (max.)

Cooling

Up to 6 °C/sec (max.)

Adjustable temperature range

4 °C to 99 °C

Temperature uniformity

55 °C ± 0.15 °C after 15 sec

Temperature control accuracy

± 0.1 °C

Heated lid

Lid temperature

30 °C to 110 °C

Contact pressure

10 kg, automated

qPCR application

Sensitivity

Detects 1 copy of target sequence in human genomic DNA

Dynamic range

10 orders of magnitude

Multiplex analysis

Up to 6-fold

Optics

Measuring principle

Fiber optic shuttle system with 8fold scanner and color modules
for excitation and emission filters

Light source

4 longlife, high-power LED’s (RGBW)

Detector

High sensitive PMT (Photo Multiplier Tube)

Color modules

  • 12 Color-, FRET- and Protein Modules
  • 6 positions inside device

Read out time

6 sec for 96 wells independent of the number of dyes

Configuration

Free configuration in device

Upgradeability

Possible without service

Control

Control

qPCRsoft or optional interface for automation software

Export function

Excel, *.csv, LIMS, qBase+, GeneIO, GenEx

Additional technical data

Weight

Approx. 30 kg

Dimension
(W x H x L)

  • Closed tray: 275 mm x 463 mm x 308 mm
  • Open tray: 275 mm x 463 mm x 420 mm
  • Electronic module: 275 mm x 186 mm x 275 mm

Recommended footprint

10 – 15 cm behind rear side of device

Interface

USB, RS232

Noise emission

45 db

Power consumption

Max. 850 W

Power supply

100 – 240 V

Warranty

  • 2 warranty on device system
  • 10 years long-term warranty of high performance optics

Parameters: color modules

Name

Excitation

Emission

Dyes* (examples)

Color module 1

470 nm

520 nm

FAMTM, Sybr®Green, Alexa488®

Color module 2

515 nm

545 nm

JOETM, HEXTM, VIC®, YakimaYellowTM

Color module 3

535 nm

580 nm

TAMRATM, DFOTM, Alexa546®, NEDTM

Color module 4

565 nm

605 nm

ROXTM, TexasRed®, Cy3.5®

Color module 5

630 nm

670 nm

Cy5®, Alexa633®, Quasar670TM

Color module 6

660 nm

705 nm

Cy5.5®, LightCycler Red®

FRET module 1

470 nm

580 nm

FAMTM (donor) / TAMRATM (acceptor)

FRET module 2

470 nm

670 nm

FAMTM (donor) / Cy5® (acceptor)

FRET module 3

470 nm

705 nm

FAMTM (donor) / Cy5.5® (acceptor)

FRET module 4

515 nm

670 nm

JOETM (donor) / Cy5® (acceptor)

FRET module 5

470 nm

605 nm

FAMTM (Donor)/ROXTM (acceptor)

Color modul Protein 1

490 nm

580 nm

SYPRO® Orange

* Yakima Yellow is registered trademark of Epoch Biosciences, Inc. Cy is a trademark of GE Healthcare. FAM, HEX, JOE, VIC, TAMRA, NED and ROX are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. SYBR, Alexa Fluor, SYPRO and Texas Red are registered trademarks of Molecular Probes, Inc. TaqMan and LightCycler are registered trademarks of Roche Group, Inc. Quasar Dyes are trademarks of Biosearch Technologies Inc. DFO™ is a trademark of Eurogentec S.A. Windows and Excel are trademarks of Microsoft Corporation.

Order Info

Order number

Description

844-00603-2

qTOWER³ auto, 220 V

Instrument system, without PC, including qPCRsoft, electronic module with 10 m cable, thermal block and detection module* for quantitative real-time PCR

844-00603-4

qTOWER³ auto, 115 V

Instrument system, without PC, including qPCRsoft, electronic module with 10 m cable, thermal block and detection module* for quantitative real-time PCR

844-00603-5

qTOWER³ auto, 110 V

Instrument system, without PC, including qPCRsoft, electronic module with 10 m cable, thermal block and detection module* for quantitative real-time PCR

* The Color or FRET modules can be ordered separately. The qTOWER 2.0 or 2.2 can be equipped with up to 6 modules.

Parameters color module

844-00520-0

Color module 1 - FAMTM, Sybr®Green, Alexa488®

844-00521-0

Color module 2 - JOETM, HEXTM, VIC®, YakimaYellowTM

844-00522-0

Color module 3 - TAMRATM, DFOTM, Alexa546®, NEDTM

844-00523-0

Color module 4 - ROXTM, TexasRed®, Cy3,5®

844-00524-0

Color module 5 Cy5®, Alexa633®, Quasar670TM

844-00525- 0

Color module 6 - Cy5.5®, LightCycler Red®

844-00526-0

FRET module 1 - FAMTM / TAMRATM

844-00527-0

FRET module 2 - FAMTM / Cy5®

844-00528-0

FRET module 3 - FAMTM / Cy5.5®

844-00529-0

FRET module 4 - JOETM / Cy5®

844-00531-0

FRET module 5 - FAMTM / ROXTM

844-00530-0

Color module Protein 1 - SYPRO® Orange

* Yakima Yellow is registered trademark of Epoch Biosciences, Inc. Cy is a trademark of GE Healthcare. FAM, HEX, JOE, VIC, TAMRA, NED and ROX are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. SYBR, Alexa Fluor, SYPRO and Texas Red are registered trademarks of Molecular Probes, Inc. TaqMan and LightCycler are registered trademarks of Roche Group, Inc. Quasar Dyes are trademarks of Biosearch Technologies Inc. DFO™ is a trademark of Eurogentec S.A. Windows and Excel are trademarks of Microsoft Corporation.

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