qTOWER 2.0/2.2

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Features

  • Quantitative real-time PCR in proven 96 well SBS standard format
  • State-of-the-art ramping rates of up to 5.5 °C/sec
  • For usage of different optical plastic ware: 0.2 ml Tubes,
    8 well strips or 96 well microplates
  • Optimized for volumes of 10 – 60 µl
  • Available with or without gradient function (max. 40 °C)
  • Patented high performance optical system with a long-term warranty of 10 years
  • Individual configuration with up to 6 different measurement channels
  • Selection out of 12 high-resolution, retrofittable color or
    FRET modules
  • High-speed scan: 6 sec. for a 96 well microplate
    (independent of the number of dyes to be measured)
  • Multilingual intuitive control and evaluation software
  • Wide variety of different evaluation methods
  • MIQE compliant

Product Info

Real-time PCR with striking design

Now, in addition to the qTOWER for rapid qPCR, the product family includes the standard real-time thermal cycler qTOWER 2.0. Featuring a striking, modern design, this system allows quantitative PCR in an established 96 well SBS standard format. The qTOWER 2.0 offers an open platform for any kind of real-time PCR plastic materials, such as 0.2 ml single tubes, 8 well strips or 96 well microplates. The high quality silver block of the qTOWER 2.0 ensures an outstanding level of temperature homogeneity of 0.2 °C along the whole block and is therefore ideally suited for all real-time PCR applications. In combination with the optional gradient function, different assays can be optimized with minimum effort. The qTOWER 2.0 is equipped with a patented, fiber-optic shuttle system for the best possible excitation and detection of a variety of known fluorescence dyes.

Silver block technology
The 96 well block of the qTOWER 2.0 is the basis for performing quantitative real-time PCR. The thermal block is made of gold-coated silver to achieve the best possible performance and maximum thermal conductivity. The resulting outstanding homogeneity and uniformity of temperature combined with state-of-the-art heating rates of up to 5.5 °C/sec and cooling rates of up to 4.0 °C/sec make the instrument the first choice for standard real-time PCR. Optionally, the qTOWER 2.2 with gradient function is available. The maximum gradient temperature range of 40 °C across 12 columns optimally prepares the instrument to establish new primer pairs. Thereby a special feature is the possibility of programming linear gradients, which not only significantly simplifies the evaluation of results, but also optimizes the whole adaptation process.

  • Quantitative real-time PCR in proven 96 standard SBS format
  • Flexible use of different optical plastic materials: 0.2 ml tubes,
    8 well strips or 96 well microplates
  • State-of-the-art ramping rates of up to 5.5 °C/sec High performance gradient function across 12 columns with a range
    of 40 °C

To avoid potential condensation and to prevent possible sample loss, the qTOWER 2.0 is equipped with a motorized heated lid. It is adjustable up to 110 °C and guarantees optimum contact pressure on the sample tubes or plates during the complete run, independent of the used consumables.

Patented fiber optical shuttle system
The qTOWER 2.0 works with 3 independent, blue, white and red, long-term stable LEDs to optimally excite the different, applicable fluorescence dyes in a wide spectral range. It ensures the highest possible quantum yield to be achieved in each real-time PCR experiment. The qTOWER 2.0 can process sophisticated multiplex experiments with up to 6 different, fluorescence-labelled probes – ranging from blue to the far-red spectral range – without any difficulty. Moreover, the patented optical system consists of a shuttle with 8 high performance fibers, which guarantee a read-out of the 96-well block within only 6 seconds – independent of the number of dyes to be measured.

  • Patented high performance optical system with 8 optical fibers and 3 LEDs
  • Optimum homogenous excitation and detection for each well
  • Read-out of a 96 well microplate within only 6 seconds - independent from the number of dyes

Each component of the high performance fiber optical system has
a 10-year long-term warranty.

Maximum flexibility
The qTOWER 2.0 can be freely configured with the available Color and FRET modules. Depending on the application, it can be adapted to either intercalating or DNA binding dyes, hydrolysis probes or even to hybridization probes (FRET probes). The system can easily be retrofitted for future use with additional so-called Color or FRET modules. This keeps the field of applications of the qTOWER 2.0 extremely flexible and easily expandable.

  • Mounting of up to 6 different Color or FRET modules
  • Use of intercalating or DNA binding dyes, hydrolysis probes and hybridization probes
  • Freely configurable color filter selection

The evaluation and control software qPCRsoft also offers the highest level of flexibility and ease of use. The logical arrangement of all tools, intuitive handling and, last but not least, the parameter-orientated memory and programming concept make the software easy to use and clear. While a cycle is in progress, the operator can easily evaluate the data of previous experiments in parallel. Based on the Ct value determination via manually or automatically adapted thresholds, the samples can be quantified absolutely or relatively and the efficiency of the PCR can be determined. In addition, the delta-delta Ct method (with or without relation to PCR efficiency) and a method for allelic discrimination, e.g. for the detection of point mutations, are available.

  • qPCRsoft: easy to use and clearly structured
  • Integrated evaluation algorithms, e.g. absolute and relative quantification, delta-delta Ct method, PCR efficiency, allelic discrimination
  • Parameter-orientated program guides
  • User management with 3 authorization levels

The qTOWER 2.0 or 2.2 convinces in every aspect and is the ideal instrument for quantitative standard real-time PCR.

Technical Data

Optical system

Principle of measurement

Top-reading fluorescence detection via 8 optical fibers with color modules for excitation and emission filters

Light source

High-power, long-life LEDs

Detector

  • CPM - channel photo multiplier
  • Highly sensitive
  • Increased SNR

Number of color modules

  • 12 available
  • 6 positions inside device

Analytical parameters

Sensitivity

1 nM FAMTM in minimal 30 µl sample volume

Read-out time

6 seconds for 96 wells independent of the number of dyes to be measured

Block capacity

96 wells for 96 well microplates,
8 well strips or individual tubes

Sample volume

10 – 60 µl

System and application parameters of the thermal cycler

Heating rate

5.5 °C/sec max

Cooling rate

4.0 °C/sec max.

Block homogeneity

± 0.2 °C

Control accuracy

± 0.1 °C

Sample block temperature

3 °C - 99 °C

Time incr./decr.

± 0.1 to 1 sec/cycle

Temperature incr./decr.

± 0.1 to 1 °C/cycle

Contact pressure

10 kg/plate, automatically

Number of programs

Not limited

Gradient

Max. 40 °C across 12 columns

Lid

  • Heated lid up to 110 °C
  • SPS technology

Other technical data

Weight

Approx. 20 kg

Dimensions
(W × H × D)

275 × 585 × 275 mm

Power supply

100 - 240 V

PC-interface

USB

Software

qPCRsoft

  • qPCRsoft
  • Control and evaluation software
  • Absolute and relative quantification
  • Delta-delta Ct method
  • Allelic discrimination
  • PCR efficiency
  • Melting curve analysis
  • MIQE compliant

Warranty

  • 2 years
  • 10 years warranty on the components of the high performance optical system

Parameters: color modules

Name

Excitation

Emission

Dyes* (examples)

Color module 1

470 nm

520 nm

FAMTM, Sybr®Green, Alexa488®

Color module 2

515 nm

545 nm

JOETM, HEXTM, VIC®, YakimaYellowTM

Color module 3

535 nm

580 nm

TAMRATM, DFOTM, Alexa546®, NEDTM

Color module 4

565 nm

605 nm

ROXTM, TexasRed®, Cy3.5®

Color module 5

630 nm

670 nm

Cy5®, Alexa633®, Quasar670TM

Color module 6

660 nm

705 nm

Cy5.5®, LightCycler Red®

FRET module 1

470 nm

580 nm

FAMTM (donor) / TAMRATM (acceptor)

FRET module 2

470 nm

670 nm

FAMTM (donor) / Cy5® (acceptor)

FRET module 3

470 nm

705 nm

FAMTM (donor) / Cy5.5® (acceptor)

FRET module 4

515 nm

670 nm

JOETM (donor) / Cy5® (acceptor)

FRET module 5

470 nm

605 nm

FAMTM (Donor)/ROXTM (acceptor)

Color modul Protein 1

490 nm

580 nm

SYPRO® Orange

* Yakima Yellow is registered trademark of Epoch Biosciences, Inc. Cy is a trademark of GE Healthcare. FAM, HEX, JOE, VIC, TAMRA, NED and ROX are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. SYBR, Alexa Fluor, SYPRO and Texas Red are registered trademarks of Molecular Probes, Inc. TaqMan and LightCycler are registered trademarks of Roche Group, Inc. Quasar Dyes are trademarks of Biosearch Technologies Inc. DFO™ is a trademark of Eurogentec S.A. Windows and Excel are trademarks of Microsoft Corporation.

Order Info

Order number

Description

844-00501-2

qTOWER 2.0

Instrument system for 220 V, without PC, including qPCRsoft, thermal block and detection module*
for the performance of quantitative real-time PCR

844-00501-4

qTOWER 2.0

Instrument system for 115 V, without PC, including qPCRsoft, thermal block and detection module*
for the performance of quantitative real-time PCR

844-00501-5

qTOWER 2.0

Instrument system for 110 V, without PC, including qPCRsoft, thermal block and detection module*
for the performance of quantitative real-time PCR

844-00502-2

qTOWER 2.2

Instrument system for 220 V with gradient function, without PC, including qPCRsoft, thermal block
and detection module* for the performance of quantitative real-time PCR

844-00502-4

qTOWER 2.2

Instrument system for 115 V with gradient function, without PC, including qPCRsoft, thermal block
and detection module* for the performance of quantitative real-time PCR

844-00502-5

qTOWER 2.2

Instrument system for 110 V with gradient function, without PC, including qPCRsoft, thermal block
and detection module* for the performance of quantitative real-time PCR

* The Color or FRET modules can be ordered separately. The qTOWER 2.0 or 2.2 can be equipped with up to 6 modules.

844-00520-0

Color module 1 - FAMTM, Sybr®Green, Alexa488®

844-00521-0

Color module 2 - JOETM, HEXTM, VIC®, YakimaYellowTM

844-00522-0

Color module 3 - TAMRATM, DFOTM, Alexa546®, NEDTM

844-00523-0

Color module 4 - ROXTM, TexasRed®, Cy3,5®

844-00524-0

Color module 5 Cy5®, Alexa633®, Quasar670TM

844-00525-0

Color module 6 - Cy5.5®, LightCycler Red®

844-00526-0

FRET module 1 - FAMTM / TAMRATM

844-00527-0

FRET module 2 - FAMTM / Cy5®

844-00528-0

FRET module 3 - FAMTM / Cy5.5®

844-00529-0

FRET module 4 - JOETM / Cy5®

844-00531-0

FRET module 5 - FAMTM / ROXTM

844-00530-0

Color module Protein 1 - SYPRO® Orange

* Yakima Yellow is registered trademark of Epoch Biosciences, Inc. Cy is a trademark of GE Healthcare. FAM, HEX, JOE, VIC, TAMRA, NED and ROX are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. SYBR, Alexa Fluor, SYPRO and Texas Red are registered trademarks of Molecular Probes, Inc. TaqMan and LightCycler are registered trademarks of Roche Group, Inc. Quasar Dyes are trademarks of Biosearch Technologies Inc. DFO™ is a trademark of Eurogentec S.A. Windows and Excel are trademarks of Microsoft Corporation.

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