innuSCRIPT Reverse Transcriptase

Features

  • ƒImproved specificity and activity in cDNA synthesis
  • Can be used at a wide range of temperatures (42° C – 55° C) ƒƒ
  • Positive test results for product sizes of 0.5 – 9 kb
  • Free of detectable endonuclease, exonuclease and RNase activity

Product Info

innuSCRIPT Reverse Transcriptase is a genetically engineered version of MMLV reverse transcriptase. Genetic engineering has made the enzyme more specific and active in cDNA synthesis— even within a broad temperature range of 42° C to 55° C. Plus, innuSCRIPT Reverse Transcriptase produces higher cDNA synthesis yields than traditional enzymes, delivering excellent results for real-time PCR. innuSCRIPT Reverse Transcriptase has been tested for cDNA synthesis using various RNA starting samples and product sizes ranging from 0.5 to 9.0 kb.

The enzyme tested negative for detectable endonu-clease, exonuclease and RNase activity.

Order Info

Order number

Quantity

845-RT-5000010

10 units (250 U)

845-RT-5000050

50 units (1.250 U)

845-RT-5000200

200 units (5.000 U)

Specifications

Concentration:

25 U/μL

10 x RT buffer:

500 mM Tris-HCl (pH 8.3 at 25 °C); 750 mM KCl; 30 mM MgCl2

DTT solution:

100 mM

Enzyme storage buffer:

20 mM Tris-HCl, pH 8.0; 0.1 mM EDTA; 1 mM DTT; 0.01% Igepal CA-630; 0.1 M NaCl; 50% Glycerol

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