LOOXSTER® Enrichment Kit

LOOXSTER<sup>®</sup> Enrichment Kit

Features

  • ƒEnriches bacterial and fungal DNA from predominantly eucaryotic DNA isolates
  • Suitable for up to 300 μg of input DNA
  • Removes over 95 % of eucaryotic DNA
  • Includes DNA cleanup

Product Info

The LOOXSTER® Enrichment Kit is a sample preparation system for enriching bacterial and fungal deoxyribonucleic acids (DNA) in a DNA isolate of predominantly eucaryotic origin. The specific affinity of the LOOXSTER® protein for non-methylated CpG dinucleotides is what produces the LOOXSTER® enrichment effect. DNA extracts containing a mixture of methylated host DNA and small quantities of double-stranded genomic bacterial or fungal DNA, are incubated with LOOXSTER® in the presence of a stringent buffer. A subsequent wash step can be used for removing unbound DNA. The enriched bacterial DNA is then eluted with the aid of an elution buffer.
The PureProve® concept: following suitable processes for reducing contamination with DNA, all system components are filled and packaged under clean-room conditions.

Order Info

Order number

Quantity

85-203-001-0010

10 reactions

Specifications

Starting material:

Up to 300 μg of predominantly eucaryotic DNA

Average yield:

  • ƒNo more than 3 μg of enriched DNA
  • The concentration of bacterial DNA in the enriched DNA depends on the ratio of eucaryotic DNA to procaryotic DNA.
  • Example: Less than 5 % of the human DNA and approx. 50 % of the bacterial DNA (E. coli) were isolated from a 100,000-fold excess of human DNA in the starting sample.

Average purity:

1.7 - 2.0

Extraction time:

Approx. 75 minutes

Downloads

Download on Request
(e.g. Manual, Short Manual)

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